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Appendix D
5/20/2009
8
7.6.2 Chlorophyll
α
from Anacystis nidulans algae, PN C6144-1MG,
ordered from Sigma. If chlorophyll from algae is not available, chlorophyll
α
from spinach may be substituted.
7.7 Quality Control Sample (QCS) – For this procedure, the QCS can be any
certified sample which is obtained from an external source. If a certified
sample is not available, then use the standard material. A solid secondary
standard from Turner Designs is used.
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SAMPLE COLLECTION, PRESERVATION, AND STORAGE
8.1 Water collected for chlα should be filtered through a Whatman GF/F glass
fiber filter (nominal pore size 0.7
µ
m), or equivalent.
8.2 Sediments collected for chlα should be sampled to a known depth and
area, or known wet weight.
8.3 Solid matrices used to determine epiphytic deposition, should not be
soluble in acetone and should be of a size to fit within a 50 ml centrifuge
tube and fit below the 40 ml mark. Mylar sheets are commonly used.
8.4 Water collected for chlα should be filtered as soon as possible. If
immediate filtration is not possible, the water samples should be kept on
ice in the dark and filtered within 24 hours.
8.5 The sample is kept frozen at -20
°
C or lower. Filter pads may be stored in
folded aluminum foil pouches. Sediments and solid matrix samples may
be stored in 50 ml polypropylene centrifuge tubes. Do NOT use
polystyrene tubes.
8.6 Frozen chlα samples should be analyzed within 4 weeks.
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QUALITY CONTROL
9.1 The laboratory is required to operate a formal quality control (QC)
program. The minimum requirements of this program consist of an initial
demonstration of laboratory capability and the continued analysis of
laboratory instrument blanks and calibration standard material, analyzed
as samples, as a continuing check on performance. The laboratory is
required to maintain performance records that define the quality of data
generated.
9.2
Initial Demonstration of Capability
9.2.1
The initial demonstration of capability (DOC) – is used to
characterize instrument performance (MDLs) and
laboratory performance (analysis of QC samples) prior to
the analyses conducted by this procedure.
9.2.2
Quality Control Sample (QCS/SRM) – When using this
procedure, a quality control sample is required to be
analyzed at the beginning and end of the run, to verify data
quality and acceptable instrument performance. If the
determined concentrations are not within
±
10% of the